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目的 探究妊娠期糖尿病(gestational diabetes mellitus, GDM)孕妇子代的DNA损伤与新生儿神经发育异常的关联。方法 选取2024年10月～2025年4月于寿光市人民医院收治的127例妊娠女性为研究对象，将75 g口服葡萄糖耐量实验(oral glucose tolerance test, OGTT)正常的43例孕妇作为对照组，将OGTT异常的84名孕妇作为观察组，再根据血糖控制情况分为两组：血糖控制正常者(观察1组，n=42)和血糖控制异常者(观察2组，n=42)。用酶联免疫吸附实验(enzyme-linked immunosorbent assay, ELISA)检测其娩出新生儿脐血中8羟基脱氧鸟苷(8-OHdG)、磷酸化组蛋白(γH2AX)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平。采用新生儿20项行为神经评分法(NBNA)评估2日龄新生儿神经发育情况。结果 观察1、2组的8-OHdG、γH2AX、MDA水平高均于对照组(P<0.05);观察2组的SOD水平低于对照组、观察1组(P<0.05);观察2组NBNA评分低于对照组、观察1组(P<0.05);8-OHdG、γH2AX、MDA水平与2日龄新生儿神经行为评分呈负相关(P<0.05)。SOD水平与2日龄新生儿神经行为评分呈正相关(P<0.05)。结论 GDM可能通过诱导氧化应激发生、造成胎儿DNA氧化损伤，进而参与子代神经行为发育的调控过程。
Abstract：Objective Association of offspring DNA damage with neonatal neurodevelopmental abnormalities in gestational diabetes mellitus(GDM).Methods A total of 127 pregnant women admitted to Shouguang People's Hospital from October 2024 to April 2025 were enrolled as subjects.Based on the 75 g oral glucose tolerance test(OGTT) results, 43 pregnant women with normal results were designated as the control group, and 84 with abnormal results as the observation group.The observation group was further stratified by glycemic control status into those with adequate control(Observation Subgroup 1,n=42) and those with inadequate control(Observation Subgroup 2,n=42).Levels of 8-hydroxy-2'-deoxyguanosine(8-OHdG),phosphorylated histone H2AX(γH2AX),malondialdehyde(MDA),and superoxide dismutase(SOD) in umbilical cord blood of neonates were measured using enzyme-linked immunosorbent assay(ELISA).Neurodevelopmental status of neonates at 2 days of age was assessed using the Neonatal 20-item Behavioral Neurological Assessment(NBNA).Results Levels of 8-OHdG,γH2AX,and MDA were significantly higher in both Observation Subgroups 1 and 2 compared with the control group(P<0.05).SOD levels were significantly lower in Observation Subgroup 2 than in both the control group and Observation Subgroup 1(P<0.05).NBNA scores in Observation Subgroup 2 were significantly lower than those in the control group and Observation Subgroup 1(P<0.05).Furthermore, levels of 8-OHdG,γH2AX,and MDA showed a significant negative correlation with the 2-day NBNA scores(P<0.05),whereas SOD levels demonstrated a significant positive correlation with the scores(P<0.05).Conclusion These findings suggest that gestational diabetes mellitus(GDM) may contribute to the regulation of offspring neurobehavioral development by inducing oxidative stress and consequent fetal oxidative DNA damage.
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基本信息:
DOI：10.16846/j.issn.1004-3101.2026.02.011
中图分类号:R714.256
引用信息:
[1]杜阳,江洪.胎儿DNA损伤在妊娠期糖尿病致新生儿神经发育异常中的作用[J].山东第二医科大学学报,2026,48(02):136-140.DOI:10.16846/j.issn.1004-3101.2026.02.011.
基金信息:
潍坊市科技发展计划项目(医学类)(项目编号：2021YX008)
2026-04-14
2026-04-14